Anti-VSIG8, CT (V-set and immunoglobulin domain containing 8)

VSIG8 protein expression was observed in the hair follicle at specific stages of the hair cycle, the oral mucosa, and the nail matrix. Expression in the hair follicle and shaft was found in the cuticle and cortex layers of the hair, where the labeling appeared intracellular and at the cell periphery. IHC analysis revealed that VSIG8 was located in the projections of the cortex between medullary cells, a poorly understood structure that is deficient in mice homozygous for the hair interior defect due to a mutation in Soat1 (Wu et al., 2010). To determine whether VSIG8 was expressed at all stages of the hair cycle or only in specific stages and anatomical sites, synchronized (wax-stripped) hair follicles (Sundberg and Silva, in press) were tested. Reactivity was restricted as we initially found in the hair follicle and hair shaft, and positive signals were limited to the late anagen and early catagen stages of the hair cycle. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:800, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.