Anti-VSIG1 (V-set and Ig Domain-containing Protein 1, Glycoprotein 34, Cell surface antigen 33) (Bio

VSIG1 (V-set and Ig domain-containing protein 1, also Glycoprotein 34 and Cell surface antigen 33) is a 55-70kD member of the JAM family of Ig-Superfamily proteins. It shows expression on gastric epithelium and testicular germ cells and likely participates in cell adhesion. Mouse VSIG1 , precursor is 407 amino acids in length. It is a type I transmembrane glycoprotein that contains a 211aa extracellular domain (ECD) (aa23-234) and a 152aa cytoplasmic region. The ECD contains one V-type (aa23-134) and one C2-type Ig-like domain (aa145-229). Over aa23-234, mouse VSIG1 is 94% and 81% aa identical to rat and human VSIG1, respectively. There is one potential alternate start site at Met108. , Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant mouse VSIG1 and recombinant rat VSIG1 is ~5ng/lane under non-reducing and reducing conditions., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.