Anti-VE-Cadherin

VE-cadherin, also known as CD144, is a type II classical cadherin and member of the cadherin superfamily of calcium-dependent cell adhesion molecules. It is composed of an N-terminal signal peptide, a pro-peptide, five extracellular cadherin repeats that bind to homotypic cadherins, a transmembrane domain, and a cytoplasmic tail that interacts with the cytoskeleton. VE-cadherin is endothelial cell-specific and is localized to intracellular junctions where it participates in vascular structure assembly. An anti-VE-cadherin antibody in combination with an anti-CD31 antibody inhibits tube formation in human umbilical vein endothelial cells (HUVECs). Knockout of Cdh5, the gene encoding VE-cadherin, in mouse embryonic stem cells prevents the formation of vessel-like structures in 11-day-old embryoid bodies. VE-cadherin is overexpressed in melanoma and breast cancer and is positively correlated with poor prognosis. Cayman's VE-Cadherin/CD144 Polyclonal Antibody can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), immunoprecipitation (IP), and Western blot (WB) applications.Synonyms: 7B4 Antigen, Cadherin-5, CD144, Vascular Endothelial Cadherin. Immunogen: Recombinant VE-cadherin peptide fragment (amino acids 259-434). Formulation: (Request formulation change), 500 µl of protein A-purified polyclonal antibody. Storage Buffer: 0.1 M Tris-glycine, pH 7.8, containing 50% glycerol, and 0.02% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ICC, IHC, IP, WB. Cross Reactivity: (+) VE-cadherin. UniProt Accession #: P33151. Origin: Animal/Rabbit.