Anti-VASP, mAb (IE273)

Contains Positive Control: Human platelet protein (500µg) (Prod. No. AG-35T-0001), supplied at 5mg/ml in SDS-stop solution (100mM NaCl, 73mM Tris/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% [v/v] glycerol, 2% [w/v] SDS, 10?g/ml bromophenol blue sodium salt). Use 5?l (25?g) per lane. Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. [Uniprot]