Anti-Vacuolar Protein Sorting 26, Homolog A (S. pombe)(VPS26A, Vacuolar Protein Sorting 26 (Vacuolar

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. , Recommended Dilutions: Peptide ELISA Titer: 1:4000, Western Blot: 1-3ug/ml ~40kD band observed in Human Kidney lysates (calculated MW of 38.2kD according to NP_004887.2). In transfected HEK293 transiently expressing VPS26A a band of approx. 40kD is observed. This band is not observed in the non-transfected HEK293. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.