Anti-UBE2A/UBE2B / Ubiquitin-conjugating enzyme E2

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. UBE2A/UBE2B antibody detects ubiquitin-conjugating enzyme E2 A and ubiquitin-conjugating enzyme E2 B, encoded by the UBE2A and UBE2B genes on chromosomes Xq24 and 5q31.1, respectively. UBE2A/UBE2B antibody is used to study these highly homologous enzymes, both members of the E2 ubiquitin-conjugating enzyme family that catalyze the transfer of ubiquitin from E1 activating enzymes to E3 ligases. Their high sequence similarity (>90%) means many antibodies recognize both proteins. UBE2A and UBE2B act in tandem with E3 ligases to ubiquitinate histones, transcription factors, and cell cycle regulators, thereby controlling DNA repair, spermatogenesis, and chromatin dynamics.Structurally, UBE2A and UBE2B contain a conserved ubiquitin-conjugating catalytic (UBC) domain with the active-site cysteine required for ubiquitin transfer. This cysteine forms a thioester bond with ubiquitin, enabling conjugation to lysine residues in substrate proteins. While UBE2A and UBE2B are highly similar, UBE2A has additional N-terminal extensions that regulate interactions with E3 ligases. Both enzymes partner with the E3 ligase Rad18 to facilitate DNA damage bypass by ubiquitinating PCNA (proliferating cell nuclear antigen), a key step in translesion DNA synthesis. Through this function, UBE2A and UBE2B maintain genome stability and promote cell survival following DNA damage.UBE2A mutations are associated with intellectual disability and neurodevelopmental disorders, underscoring its role in neuronal maintenance and protein quality control. UBE2B, on the other hand, is essential for male fertility, with knockout mice exhibiting azoospermia and meiotic arrest due to defective histone ubiquitination during spermatogenesis. Their overlapping and specialized functions highlight evolutionary conservation and diversification of ubiquitin signaling. Researchers use UBE2A/UBE2B antibody to track their expression in reproductive, neurological, and DNA repair contexts.Clinically, aberrant regulation of UBE2A/UBE2B is implicated in cancer, where altered ubiquitination disturbs cell cycle control and DNA repair fidelity. Overexpression in tumors enhances tolerance to genotoxic stress, while reduced activity promotes genomic instability. Targeting their interactions with E3 ligases is being explored therapeutically. Experimental applications of UBE2A/UBE2B antibody include western blotting for expression profiling, immunohistochemistry in testicular and brain tissue, and immunoprecipitation to analyze ubiquitination complexes. NSJ Bioreagents provides UBE2A/UBE2B antibody to enable research in DNA repair, neurobiology, reproduction, and cancer biology.