Anti-TWIST1 (Class A Basic Helix-loop-helix Protein 38, bHLHa38, H-twist, BHLHA38, TWIST, Twist-rela

Twist1 (Twist-related protein 1? also HTwist and bHLHa38) is a 24-27kD class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist1 induces fatty acid oxidation via PGC1 a and CPT1, and promotes MCP1 and TNFa secretion by adipocytes. In epithelium, Twist1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to mesenchymal transition that can lead to cancer. Human Twist 1 is 202aa in length. It contains a bHLH domain (aa109-164) with an embedded DNA binding motif. Twist1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one 7aa insertion after 135aa, and a second 7aa insertion after139aa. A third unrelated variant shows a four Gly insert after Gly92. Full length human Twist1 shares 98% aa identity with mouse Twist1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.