Anti-Tumor Necrosis Factor alpha (TNFa) (Biotin)

TNF-a, designated TNFSF1A, is a trimeric glycoprotein active in both membrane bound and secreted forms. TNF-a is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-a binds to TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.