Anti-TTK (TTK Protein Kinase, Dual Specificity Protein Kinase TTK, ECSTY Kinase, ESK, FLJ38280, hMPS

TTK (Dual specificity Thr/Tyr kinase, also PYT and hMps1) is a 97-105kD member of the Ser/Thr protein kinase family of enzymes. It is expressed in rapidly proliferating tissues and cell lines, and participates in cell cycle regulation. In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation. Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2. Human TTK is 857 (aa) in length and contains one protein kinase domain (aa525-791). Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686. Apoplications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.