Anti-Tryptase, beta2 (TPSB2, MCPT6, Mast Cell Protease 6)

As major neutral proteases present in the mast cells, tryptases function as mediators in the pathogenesis of asthema and other allergic and inflammatory disorders. Tryptase, beta2 (TPSB2) consists of a signal peptide (residues 1 to 21), a pro peptide (residues 22 to 31) and a mature chain (residues 32 to 275). The amino acid sequence of mouse TPSB2 is 88%, 78%, 76%, and 73% identical to that of mouse, human (TPSB2 and TPSAB1), chimpanzee, and canine. Applications: Suitable for use in Immunoprecipitation, ELISA and Neutralization. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 25ug/ml immunoprecipitates recombinant mouse Tryptase, beta 2 from conditioned cell culture medium. , Neutralization: Measured by its ability to neutralize Recombinant Mouse Mast Cell Protease­6/Mcpt6 (1ug/ml) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10uM). The Neutralization Dose (ND50) is typically 3ug/ml., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.