Anti-TRIM32 / Tripartite motif-containing protein 32

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. TRIM32 antibody detects Tripartite motif-containing protein 32, an E3 ubiquitin ligase that regulates protein turnover, muscle differentiation, and cellular signaling. TRIM32 is part of the TRIM family of RING finger proteins that mediate ubiquitin-dependent degradation and transcriptional regulation. The TRIM32 antibody is widely used in muscle biology, neurodegeneration, and oncology research to study ubiquitination, differentiation, and signaling regulation.TRIM32 is encoded by the TRIM32 gene located on human chromosome 9q33.1. The protein is approximately 653 amino acids long and contains a RING domain, one or two B-box domains, and a coiled-coil region that mediates protein-protein interactions, followed by a C-terminal NHL repeat domain responsible for substrate recognition. TRIM32 localizes to the cytoplasm, nucleus, and occasionally to cytoskeletal structures, depending on cell type and activity state.The TRIM32 antibody detects an 80 kilodalton protein by western blot and shows punctate cytoplasmic staining under immunofluorescence microscopy. TRIM32 functions as an E3 ubiquitin ligase that targets a variety of substrates involved in differentiation, including actin regulators, transcription factors, and signaling molecules. In skeletal muscle, TRIM32 promotes myogenesis by ubiquitinating inhibitors of differentiation such as NDRG2 and MyoD regulators. In neurons, TRIM32 modulates synaptic plasticity and axonal growth by regulating cytoskeletal remodeling and degradation of synaptic proteins.Mutations in TRIM32 cause Limb-Girdle Muscular Dystrophy type 2H and Bardet-Biedl syndrome, disorders characterized by muscle weakness, retinal degeneration, and developmental defects. These mutations often impair E3 ligase activity or disrupt substrate binding, leading to accumulation of misfolded or damaged proteins. TRIM32 is also implicated in tumorigenesis, acting as either a tumor suppressor or oncogene depending on cellular context. Its ubiquitination of MYC and p53 pathway components links it directly to cancer cell proliferation and apoptosis.Because TRIM32 integrates ubiquitin signaling with cellular differentiation and homeostasis, it serves as an essential node in protein quality control and tissue development. NSJ Bioreagents provides a validated TRIM32 antibody optimized for western blot, immunofluorescence, and ubiquitination assays, supporting research into muscle physiology, neurodegeneration, and cancer signaling.