Anti-TREML2 (Triggering Receptor Expressed on Monocytes-Like Protein 2, TREM-Like Transcript 2, TLT-

Mouse Trem-like transcript 2 protein is a 34kD (predicted for unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Mouse TLT-2 is synthesized as a 329aa precursor that contains a 24aa signal sequence, a 246aa extracellular domain (ECD), a 21aa transmembrane segment, and a 38aa cytoplasmic tail. Within the ECD are an Ig-like V-type domain aa25-126 and one potential site for N-linked glycosylation. Mature mouse TLT-2 shares 49aa sequence identity with mature human TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines. TLT-2 is also expressed constitutively on CD8+ T cells. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 10e6 cells, Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.