Anti-TNFA (Tumor Necrosis Factor, Cachectin, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Mem

TNF-alpha is a homotrimeric 17kD protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways. TNF-alpha was originally described as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-alpha is found to be a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines leading to expression of a wide variety of cytokines. The pro-inflammatory properties of TNF-alpha play a central role in several auto-immune diseases such as rheumatoid arthritis and inhibition by neutralizing molecules have been shown to be beneficial in patients. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: stains soluble TNF bound to TNF receptors. Cells were fixed in PBA containing 0.2% formaldehyde, Western Blot: A reduced sample treatment and 15% SDS-Page was used. The band size observed is 17kD, Immunohistochemistry (Paraffin): Tissue sections were fixed in 5% formalin embedded in paraffin and cut into 2um sections, Immunohistochemistry (Frozen): 6-um tissue sections were fixed in acetone for 10min, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.