Anti-TNF alpha-induced Protein 3 (A20, TNFAIP3, OTUD7C, TNFA1P2)

A20, also called TNF alpha­induced protein 3 (TNFAIP3), is a cytoplasmic zinc finger protein that inhibits NF kappa B activity and tumor necrosis factor­mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF­induced NF kappa B­dependent gene expression by interfering with RIP­ or TRAF2­mediated transactivation signaling. A20 contains an N­terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor­interacting protein (RIP), thus mediating distinct regulatory effects in the down­regulation of NF kappa B signaling. Applications: Suitable for use in ELISA and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8­25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.