Anti-TMED1 / Transmembrane emp24 domain-containing protein 1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. TMED1 antibody detects transmembrane emp24 domain-containing protein 1, a member of the p24 family of cargo receptors that regulate protein trafficking between the endoplasmic reticulum (ER) and Golgi apparatus. TMED1 participates in vesicle formation and quality control of secretory and membrane proteins, ensuring that correctly folded proteins are transported while misfolded ones are retained or degraded. The p24 family functions as part of the COPI and COPII coat protein systems, playing critical roles in maintaining organelle integrity and trafficking efficiency. The TMED1 antibody is an important reagent for studying vesicular transport, protein sorting, and ER-Golgi dynamics in various cell types.TMED1 is encoded by the TMED1 gene located on human chromosome 19p13.3. The protein comprises a luminal GOLD (Golgi dynamics) domain, a single transmembrane helix, and a short cytoplasmic tail that interacts with coatomer components. TMED1 has been implicated in the trafficking of toll-like receptors and other immune-related proteins, highlighting its role in innate immune signaling. In the Golgi network, TMED1 contributes to the formation of transport vesicles and sorting of glycoproteins, making it a crucial element in secretory pathway organization.Through use of the TMED1 antibody, researchers have identified strong expression in the Golgi apparatus and ER exit sites, where TMED1 co-localizes with other p24 family members such as TMED2, TMED4, and TMED10. Western blot typically reveals a band near 25-27 kDa corresponding to the full-length protein. Immunofluorescence labeling with this antibody provides a distinct perinuclear staining pattern consistent with Golgi localization. TMED1 expression has been linked to protein homeostasis, and its dysregulation may contribute to cellular stress responses and unfolded protein response activation.Recent studies suggest TMED1's involvement in autophagy and viral protein processing, expanding its relevance beyond classical ER-Golgi transport. Knockdown or knockout experiments have shown altered secretion of select cytokines and membrane receptors, emphasizing its importance in vesicle biogenesis and immune communication. NSJ Bioreagents offers a validated TMED1 antibody suitable for western blot, immunocytochemistry, and immunofluorescence applications. This antibody supports detailed exploration of membrane trafficking mechanisms, Golgi organization, and the molecular pathology of secretion-related diseases.