Anti-TLR5 (Toll-like Receptor 5, Toll/Interleukin-1 Receptor-like Protein 3, TIL3)

TLR5 is an ~100kD type I transmembrane glycoprotein of the Toll-like receptor family. It is expressed on mucosal epithelia in the gastrointestinal tract, airways, and other areas of potential bacterial contact and recognizes bacterial flagellin. Expression is also reported on monocytes, immature dendritic cells, and CD4+ T lymphocytes. The region of human TLR5 used as an immunogen includes the first 2 of 16 leucine-rich repeats and shares 72% aa identity with mouse and rat TLR5. This region is outside of the flagellin binding region. Flagellin engagement induces dimerization and intracellular signaling via MyD88. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug labels 10e6, Immunohistochemistry: 8-25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.