Anti-TINAG (Tubulointerstitial Nephritis Antigen, TIN-Ag)

TIN-Ag (Tubulointerstitial nephritis antigen) is a 48-58kD glycoprotein member of the peptidase C1 family of molecules. It is secreted by renal tubule epithelium and (presumably) small intestine columnar epithelium. TIN-Ag is an integral component of the renal tubule basement membrane (BM), and appears to promote proper BM matrix organization, block laminin polymerization, and serve as a receptor for the epithelial integrins a3b1 and avb3. Epithelial dissociation from the BM is associated with an epithelial-to-mesenchymal transition. Human TIN-Ag is synthesized as a 476aa preproprecursor. It contains a 19aa signal sequence, a 30aa furin-cleavable prosegment (aa20-49), and a 427aa mature region (aa50-476). Within the mature region is an SMB domain (aa61-106), one vWFC domain (aa119-154), and a nonenzymatic peptidase C1A region (aa218-466). Multiple splice variants are possible, and potential isoforms may show an alternative start site at aa322, or a combined deletion of aa119-169 plus 209-300, or a combination of an alternative start site at aa19 coupled to a 13aa substitution for aa209-476. Over aa20-476, human proTIN-Ag shares 86aa identity with mouse proTIN-Ag, over the mature region (aa50-476), human TIN-Ag shares 88aa identity with mouse TIN-Ag. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.