Anti-TIMM9 / Tim9

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. TIMM9 antibody detects Mitochondrial import inner membrane translocase subunit Tim9, a chaperone essential for importing hydrophobic carrier proteins into the mitochondrial inner membrane. Encoded by the TIMM9 gene on chromosome 14q24.3, this small protein is part of the TIM9-TIM10 complex that guides precursor proteins through the mitochondrial intermembrane space to their insertion sites. TIMM9 maintains protein folding stability during transit and prevents aggregation of hydrophobic substrates prior to membrane integration.Structurally, TIMM9 is an 89-amino-acid protein of approximately 10 kilodaltons containing twin CX3C motifs that coordinate zinc ions to stabilize its characteristic hexameric structure. Within the intermembrane space, TIMM9 forms a heterohexameric complex with TIMM10 or TIMM10B, providing a soluble chaperone system that transfers imported precursors from the TOM complex (translocase of the outer membrane) to the TIM22 complex for membrane insertion.The TIMM9 antibody is widely used in mitochondrial import, bioenergetics, and protein trafficking research to study mitochondrial assembly, carrier translocation, and protein folding. Western blot analysis detects a 10 kilodalton band corresponding to TIMM9, while immunofluorescence demonstrates mitochondrial localization colocalizing with markers such as TOM20 and COX IV. This antibody is valuable for analyzing mitochondrial protein import efficiency and intermembrane space chaperone dynamics.Functionally, TIMM9 is essential for the import of metabolite carrier proteins, including members of the ADP/ATP translocase and phosphate carrier families. Loss of TIMM9 disrupts carrier protein biogenesis, resulting in impaired mitochondrial respiration and metabolic deficiency. Defects in TIMM9 expression or complex formation have been implicated in mitochondrial encephalopathies and neurodegenerative diseases characterized by energy failure and oxidative stress. The TIMM9 antibody provides a sensitive reagent for evaluating mitochondrial import machinery integrity and studying molecular chaperone systems that maintain organelle function. NSJ Bioreagents validates this antibody for its applications, ensuring specificity and reproducibility for mitochondrial protein import studies.