Anti-Tim3 (T Cell Immunoglobulin and Mucin Domain-containing 3, T Cell Immunoglobulin Mucin 3, TIM3,

TIM-3 has been identified as a Th1 specific marker initially due to expression on activated and polarized Th1 cells and absence on naïve T cells. It has also been found on CD8+ T cells, Th17, Tregs , monocytes and dendritic cells. As a transmemebrane protein, TIM-3 is also referred to as 'T cell immunoglobulin and mucin-like domain containing protein -3.' Enhanced surface expression on CD4+ and CD8+ cells in the CNS are indicative of disease in EAE, murine models of MS. TIM-3 mediated down regulation of Th1 immune responses and induction of immune tolerance illustrate a potential key role for TIM-3 in immunoregulation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.5-1ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.