Anti-TIM-4, ID (T-cell Immunoglobulin and Mucin Domain-containing Protein 4, TIMD-4, Spleen, Mucin-c

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, Immunocytochemistry: 10ug/ml, Immunofluorescence: 20ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Jurkat Cell Lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.