Anti-TIGAR (Tp53-Induced Glycolysis and Apoptosis Regulator, Chromosome 12 Open Reading Frame 5, C12

TIGAR a 270aa, p53-inducible protein is known an isomerase belonging to phosphoglycerate mutase family. It is a part of the p53 tumor suppressor pathway that regulates glycolysis and protects against oxidative stress. Report shows that it has sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2, 6-bisphosphate and blocks glycolysis thereby directing the pathway into the pentose phosphate shunt. Over expression of TIGAR in mouse and human cells resulted in inhibition of glycolysis, as well as a decrease in intracellular reactive oxygen species (ROS) and provides some protection from DNA damage-induced apoptosis in a cell type-specific manner. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-4ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.