Anti-TICAM1 (TIR Domain-containing Adapter Molecule 1, TICAM-1, Proline-rich, Vinculin and TIR Domai

TRIF (TIR domain-containing adaptor inducing IFN-b, also TICAM1) is a 105-110kD cytoplasmic adaptor molecule that mediates Toll receptor signaling. It is widely expressed and associates with both TLR3 and TLR4. Relative to TLR3, TRIF appears to activate IRF-3, -4, and -7, as well as NFkB and FADD. Its action on FADD is through RIP1, and this induces apoptosis. Human TRIF is 712aa in length and contains three TRAF6 bonding motifs (aa84-91, 248-255 and 299-309), one TIR domain (aa390-460), a Pro-rich region (aa614-678), and an overlapping RHIM domain (aa661-699). The molecule is reported to form a homodimer. There are multiple potential isoform variants. One shows a 23aa substitution for aa31-162 accompanied by a Pro substitution for aa633-660, a second shows a 44aa substitution for aa352-712, and a third shows a 38aa substitution for aa271-712. Over aa29-204, human TRIF shares 62% aa identity with mouse TRIF. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot:0.25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.