Anti-THOC4 (ALY, Transcriptional Coactivator Aly/REF)

mRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing of mRNAs and the packaging of mRNAs in the form of mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4/ALY via its interaction with the splicing factor UAP56, forming a large complex termed transcription-export complex (TREX) (1,2,5). THOC4/ALY then directly interacts with NXF1/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus to the cytoplasm (1-3,6). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence(Immunocytochemistry): 1:400, Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.