Anti-TFB2M / Transcription factor B2, mitochondrial

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. TFB2M antibody detects Transcription factor B2, mitochondrial, encoded by the TFB2M gene on chromosome 1q44. TFB2M antibody is commonly used in research on mitochondrial transcription and energy metabolism. TFB2M functions as a key initiation factor for mitochondrial RNA polymerase (POLRMT), helping to promote promoter melting and start site recognition during transcription initiation. By supporting expression of mitochondrial genes encoded in mtDNA, TFB2M is essential for the assembly of oxidative phosphorylation complexes and overall cellular respiration. Expression is detected in many tissues, with particularly high levels in energy-demanding organs such as heart, brain, and skeletal muscle.Structurally, TFB2M belongs to the rRNA adenine dimethyltransferase family but has largely lost enzymatic activity. Instead, it has adapted to act as a specialized transcription factor within mitochondria. TFB2M contains an S-adenosylmethionine (SAM)-dependent methyltransferase-like fold, which has been repurposed for interactions with POLRMT and promoter DNA. Its ability to stabilize the open complex at promoters distinguishes it from its paralog TFB1M, which primarily functions as a mitochondrial rRNA methyltransferase. This divergence highlights the specialization of mitochondrial transcription factors for different roles.Functionally, TFB2M is indispensable for mitochondrial gene expression. Knockdown of TFB2M severely impairs transcription initiation, leading to reduced synthesis of mtDNA-encoded proteins and respiratory chain dysfunction. TFB2M is also required for proper replication primer formation, as transcription of certain promoter regions overlaps with origins of replication. By coupling transcription with replication, TFB2M ensures faithful maintenance of mitochondrial genome integrity. Researchers use TFB2M antibody to study mitochondrial transcriptional regulation, energy metabolism, and disease mechanisms involving mitochondrial dysfunction.Clinically, defects in mitochondrial transcription, including reduced TFB2M activity, are associated with mitochondrial diseases characterized by encephalomyopathy, cardiomyopathy, and metabolic failure. Altered TFB2M expression has also been linked to cancer, where mitochondrial metabolism is frequently reprogrammed. Elevated TFB2M expression supports high respiratory activity in certain tumor cells, while reduced expression can drive metabolic remodeling toward glycolysis. These associations highlight the clinical importance of TFB2M as a mitochondrial regulator. NSJ Bioreagents supplies TFB2M antibody as a valuable tool for investigating mitochondrial transcription and its roles in health and disease.Experimentally, TFB2M antibody is used in western blotting to detect the ~45 kDa protein, in immunofluorescence to visualize mitochondrial localization, and in immunohistochemistry to study tissue-specific expression. Immunoprecipitation with TFB2M antibody enables mapping of POLRMT transcription complexes. These applications facilitate analysis of mitochondrial transcription machinery and related disease processes.