Anti-TFAP4 / Transcription factor AP-4

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. TFAP4 antibody detects Transcription factor AP-4, a basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor that regulates cell proliferation, differentiation, and apoptosis. The UniProt recommended name is Transcription factor AP-4 (TFAP4). This protein binds to E-box DNA motifs (CAGCTG) to modulate gene expression involved in developmental signaling and oncogenic transformation.Functionally, TFAP4 antibody identifies a 338-amino-acid nuclear transcription factor that acts as both an activator and repressor of transcription. TFAP4 dimerizes through its leucine zipper domain and binds to specific promoter sequences, influencing the expression of genes controlling cell cycle progression, epithelial-to-mesenchymal transition (EMT), and differentiation. It regulates genes such as MYC, CDKN1A (p21), and CCND1, linking growth control to transcriptional responses.The TFAP4 gene is located on chromosome 16p13.3 and is broadly expressed in epithelial and immune cells. TFAP4 functions downstream of MYC as a key mediator of its transcriptional program. In oncogenesis, TFAP4 promotes proliferation, invasion, and metastasis by activating EMT-related transcription factors such as SNAIL and repressing cell adhesion molecules like E-cadherin. Conversely, under stress conditions, TFAP4 can trigger apoptotic pathways through p53-dependent transcriptional activation.TFAP4 also participates in development by regulating neuronal and epithelial differentiation. Its activity is modulated by post-translational modifications including phosphorylation and acetylation, which influence its DNA-binding affinity and cofactor recruitment. Dysregulation of TFAP4 expression has been linked to various cancers, including colorectal, breast, and gastric cancer, where it drives metastasis and stem-like phenotypes.TFAP4 antibody is widely used in transcriptional regulation, cancer biology, and developmental studies. It is suitable for western blotting, immunohistochemistry, and chromatin immunoprecipitation to detect TFAP4 protein levels and promoter occupancy. This antibody supports investigations into MYC signaling, cell cycle regulation, and transcriptional reprogramming. In oncology, TFAP4 detection provides insights into tumor progression and epithelial plasticity.Structurally, TFAP4 contains a bHLH-LZ domain for DNA binding and dimerization, along with transactivation and repression regions that recruit chromatin-modifying complexes. NSJ Bioreagents provides TFAP4 antibody reagents validated for use in transcriptional control, oncogenic signaling, and developmental regulation research.