Anti-TCR, alpha, beta, V beta1 (T Cell Receptor), Phycoerythrin conjugated, clone 5K82

This antibody precipitates a CD3-associated heterodimer of Mr 90kD (two bands of Mr 40kD and 50kD upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 34kD and 29kD from antibody precipitates. In the chicken, two distinct subpopulations of alpha/beta T cells appear in the thymus subsequent to the appearance of gamma/delta T cells. These subpopulations, originally denoted as TCR2 and TCR3,1-3 arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vb families, Vb1 and Vb2, respectively. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74±2%) or CD8 (26±4%). TCR2+ cells in the spleen also express either CD4 (37±1%) or CD8 (64±4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2.1-3 This observation led to the demonstration that 71±6% of the TCR1(gamma/delta)+ cells in the spleen express the CD8 marker. Applications:  Flow cytometry 1,  Immunohistochemistry (acetone-fixed, frozen sections),  In ovo suppression of the development of TCR-Vb1 cells 5,6,  Immunoprecipitation 1, Recommended Dilution: Flow Cytometry: < 0.2ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.