Anti-TCR, alpha, beta (T Cell Receptor), FITC conjugated, clone 5K25

The alpha/beta TCR is expressed on T lymphocytes of all mouse strains tested. Plate-bound or soluble antibody activates a/b TCR-bearing T cells. In vitro and in vivo, this antibody can induce immature thymocytes to undergo apoptosis. In vivo administration of this antibody has been shown to deplete a/b TCR-bearing cells to near completion and prevent graft rejection. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: <2ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.