Anti-TAF5 / TATA-box binding protein-associated factor 5

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. TAF5 antibody recognizes TATA-box binding protein-associated factor 5, a central component of the transcription factor IID (TFIID) complex encoded by the TAF5 gene on chromosome 10q24.32. As part of the basal transcription machinery, TAF5 ensures accurate initiation of transcription by RNA polymerase II. The TFIID complex is composed of TATA-binding protein (TBP) and TBP-associated factors (TAFs), and TAF5 serves as a scaffold protein that stabilizes multiple TAF interactions. This stability is necessary for promoter recognition, recruitment of RNA polymerase II, and subsequent transcription initiation across thousands of protein-coding genes.Structurally, TAF5 contains WD40 repeat motifs that fold into a beta-propeller domain, enabling diverse protein-protein interactions. Within TFIID, TAF5 interacts with TAF6L and TAF9, forming a structural core required for TFIID integrity. These associations support the assembly of the preinitiation complex and facilitate recruitment of coactivators and chromatin remodelers. Without TAF5, transcription initiation collapses, highlighting its indispensable role in gene regulation.TAF5 has also been studied in the context of development and disease. Knockout studies in model organisms demonstrate embryonic lethality, underscoring its essential function. Altered TAF5 expression has been observed in cancer, where transcriptional dysregulation contributes to uncontrolled proliferation. Variants affecting TAF5 or other TAF components are also linked to neurodevelopmental disorders, reinforcing the idea that precise transcriptional control is vital for normal brain development and function. Because of these roles, TAF5 antibody is a critical reagent for dissecting transcriptional regulation in both health and disease.Research applications of TAF5 antibody span molecular biology and epigenetics. It is widely used in western blotting to monitor TFIID subunit levels, in chromatin immunoprecipitation (ChIP) to examine promoter occupancy, and in immunofluorescence to visualize nuclear localization. Studies employing TAF5 antibody have helped map transcriptional networks, revealing how TFIID coordinates with Mediator and other coactivator complexes. These insights are crucial for understanding global transcriptional responses to developmental cues and environmental stress.TAF5's importance as a molecular scaffold extends beyond TFIID. Recent studies suggest that TAF5 may contribute to specialized transcriptional programs in certain tissues by influencing alternative TFIID assemblies. Its dynamic regulation makes it a potential target for modulating transcription in disease contexts. NSJ Bioreagents supplies TAF5 antibody to facilitate research into transcriptional initiation, chromatin remodeling, and the molecular mechanisms of gene regulation.