Anti-Syntaxin-binding protein 1 (Syntaxin-BP1, p67, Munc18-1, N-Sec1, Unc18A)

STXBP1 (Syntaxin-binding protein 1, also p67, Munc18-1, N-Sec1 and Unc18A) is a 67kD member of the SNARE-interacting Sec1/Unc18/STXBP family of proteins. It is expressed in a variety of cells, presumably in those that exhibit granule exocytosis, and likely participates in (synaptic) granule release. Ligands for STXBP1 include STX1A, 2 and 3, and it is possible that STXBP1 both suppresses STX activity, and serves as an anchor for STX-mediated vesicle fusion. Human STXBP1 is 594aa in length. It contains one SEC1 domain (aa41-575). Phosphorylation at Ser313 may stimulate granule release. There is one splice variant that shows a 28aa substitution for aa576-594. This is restricted to neural tissue. Over aa 80-226, human and , mouse STXBP1 are absolutely identical in amino acid sequence. , , Applications: , Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml, Western Blot: 1.0ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.