Anti-SYNJ2 / Synaptojanin 2

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SYNJ2 antibody detects Synaptojanin-2, a phosphoinositide phosphatase that regulates endocytosis, membrane trafficking, and cytoskeletal remodeling. Encoded by the SYNJ2 gene on chromosome 6q25.3, this protein belongs to the inositol polyphosphate 5-phosphatase family and contains a Sac1-like domain, a 5-phosphatase catalytic domain, and a proline-rich region for binding endocytic adaptor proteins. By hydrolyzing phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3), SYNJ2 controls membrane curvature, vesicle uncoating, and receptor recycling. It plays a pivotal role in clathrin-mediated endocytosis and growth factor signaling regulation.SYNJ2 is expressed in many tissues, with particularly high levels in the brain, liver, and kidney. It localizes to endocytic vesicles, the plasma membrane, and cytoplasmic puncta associated with actin filaments. Through its phosphatase activity, SYNJ2 terminates phosphoinositide-dependent signaling, facilitating the disassembly of endocytic complexes. It interacts with endophilins, amphiphysin, and dynamin to coordinate vesicle fission and cargo retrieval. In addition, SYNJ2 participates in insulin signaling and receptor recycling in adipocytes and hepatocytes, linking membrane trafficking to metabolic regulation.The SYNJ2 antibody is widely used in neuroscience, cell biology, and cancer research to study phosphoinositide metabolism and endocytosis. Western blot analysis typically identifies a 170 kilodalton band corresponding to full-length Synaptojanin-2, while immunofluorescence reveals punctate staining along endocytic vesicles and actin-rich regions. Dysregulated SYNJ2 expression has been associated with enhanced tumor cell migration and invasion, particularly in breast and prostate cancers, where it drives actin remodeling and lamellipodia formation through PI3K signaling modulation.Functionally, SYNJ2 acts at the intersection of signaling and membrane dynamics, ensuring timely lipid turnover and vesicle recycling. It also contributes to synaptic vesicle endocytosis, influencing neuronal plasticity and receptor availability. The SYNJ2 antibody supports these studies by providing precise detection of Synaptojanin-2 across multiple tissue types. NSJ Bioreagents supplies this antibody validated for its applications, enabling researchers to investigate the molecular mechanisms of phosphoinositide metabolism and signal termination.