Anti-STAT5b (Signal Transducers and Activators of Transcription 5b)

Applications:, Western Blotting: 0.5-2mg/ml detects STAT5B (doublet of 90kD and 92kD) in, human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4ug immunoprecipitates STAT5B from A431 carcinoma cells and a previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500ug protein) was incubated with 4ug of anti-STAT 5B Immunecomplexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5ul of 2-mercaptoethanol per 100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.