Anti-STAT5a,b (Signal Transducers and Activators of Transcription 5a,b)

STAT (signal transducers and activators of transcription) proteins can be selectively phosphorylated on tyrosine residues by Jak family kinases and some receptors with tyrosine kinase activity. The STAT proteins are highly conserved in their amino termini but have divergent carboxy termini, which are thought to be essential for their selective activity. STAT5 can be activated by a variety of agents including prolactin, IL-2, IL-3, IL-7, IL-15, growth hormone and erythropoietin. In peripheral blood lymphocytes (PBL), IL-2 rapidly activates STAT5 while phytohemagglutinin activates both STAT3 and STAT5. Evidence indicates that both STAT5A and STAT5B are activated in PBL in vivo. Data show that either Tyr-392 or Tyr-510 of the IL-2Rb is required for IL-2 dependent activation of STAT5. JAK1 and JAK3 may activate each STAT5 isoform by phosphorylation of a single tyrosine residue. Applications: Suitable for use in Antibody Supershift and Electrophoretic Mobility Shift Assay. Not recommended for Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Antibody Supershift: An Independent laboratory showed that Anti-STAT5 (A&B) supershifted IL-2 induced complexes in both fresh and preactivated peripheral blood lymphocytes. This antibody also supershifted the complexes induced by IL-2, IL-7 and IL-15, but not those induced by IL-4 and IL-13 in fresh peripheral blood lymphocytes. The specificity of the reaction was confirmed by competition with the immunogenic peptide (Lin, et al., 1995) Experimental conditions must be optimized to achieve the best supershift banding pattern (see Lin, et al., 1993). , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.