Anti-SRPK3 (Ser/Arg-rich Protein Specific kinase 3, MSSK1, Muscle-specific Serine Kinase 1, STK23, S

SRPK3 (Ser/Arg-rich protein specific kinase 3), also called MSSK-1 (muscle-specific serine kinase 1) or STK23 (Ser/Thr kinase 23) is a 59kD (predicted) cytoplasmic member of the CMGC Ser/Thr protein kinase family. SRPK3 is principally expressed in heart and skeletal muscle. SRPKs selectively phosphorylate Ser on RS domain-containing proteins, initiating mRNA splicing and maturation. The 533 amino acid (aa) human SRPK3 contains a split kinase domain (aa79-292 and 347-531) separated by a hinge region. A 491aa isoform shows an alternate translation start site at aa43. Within the region used as an immunogen, human SRPK3 shares 87% and 86% aa identity with mouse and rat SRPK3, respectively. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 8-25ug/ml, IOptimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.