Anti-SPT6 / SUPT6H / Transcription elongation factor SPT6

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SPT6 antibody detects Transcription elongation factor SPT6, encoded by the SUPT6H gene located on chromosome 17q11.2. SPT6 is a conserved histone chaperone and transcription elongation factor that couples RNA polymerase II (Pol II) activity to chromatin reassembly. It plays essential roles in transcription elongation, mRNA processing, and maintenance of chromatin integrity during gene expression. SPT6 is expressed in most cell types, with high levels in proliferating tissues and the central nervous system, where active transcription and chromatin remodeling are continuous.Structurally, SPT6 is a large nuclear protein (~170 kDa) containing tandem SH2 domains at the C-terminus that mediate direct binding to the phosphorylated C-terminal domain of RNA Pol II. The protein also includes acidic and basic regions that interact with nucleosomes, histones H3/H4, and elongation factors. SPT6 belongs to the transcription elongation factor family conserved across eukaryotes and forms complexes with other regulators such as IWS1, PAF1C, and FACT to coordinate chromatin structure with RNA synthesis.Functionally, SPT6 promotes nucleosome reassembly behind the elongating RNA polymerase and prevents cryptic transcription initiation within coding regions. It also regulates histone modifications, such as H3K36 methylation, by recruiting SETD2 and other histone modifiers during elongation. In addition, SPT6 influences mRNA splicing and 3'-end processing by stabilizing Pol II on gene bodies. Through its dual role as a histone chaperone and elongation factor, SPT6 ensures faithful transcriptional output and epigenetic stability.Loss or dysregulation of SPT6 disrupts transcription elongation and chromatin organization, leading to genomic instability and aberrant gene expression. Mutations have been associated with developmental defects, cancer, and neurodegenerative disorders. SPT6 also participates in transcription-coupled DNA repair and chromatin restoration after stress. Pathway associations include RNA polymerase II transcription, chromatin remodeling, and epigenetic regulation. During development, SPT6 expression supports neuronal differentiation and transcriptional fidelity in proliferating cells.The SPT6 antibody from NSJ Bioreagents is an excellent reagent for research into transcription regulation, chromatin maintenance, and epigenetic control.