Anti-SPP2 (Secreted Phosphoprotein 2, SPP24, Secreted phosphoprotein 24)

Spp24 encodes a 24kD secreted phosphoprotein that shares sequence homology with members of the cystatin family of thiol protease inhibitors and is expressed in bone, as is cystatin. Thiol proteases are known to be involved in bone turnover, and Spp24 may function as an inhibitor. The deduced 211aa protein contains a 29aa signal peptide, followed by a cystatin-like domain, a region of phosphorylated serines, and an arginine-rich C-terminal domain. mRNA dot blot analysis detected highest SPP2 expression in adult and fetal liver, with much weaker expression in adult kidney. The array used for the expression study did not contain bone mRNA. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml, Immunohistochemistry(Paraffin): 5-20ug/ml, Immunocytochemistry: 5-20ug/ml, Optimal dilutions to be determined by the researcher. Positive Control (Supplied): 365158A: SPP2, Positive Control, Suppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)., DAB: 10ul/well, ECL: 5ul/well, Store at -20°C. Stable for 6 months after receipt. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.