Anti-SNAP25 / Synaptosomal-associated protein 25

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SNAP25 antibody detects Synaptosomal-associated protein 25, a key component of the SNARE complex responsible for synaptic vesicle docking and neurotransmitter release. SNAP25 is essential for calcium-triggered exocytosis in neurons and neuroendocrine cells, making it a crucial mediator of synaptic transmission. The SNAP25 antibody is widely used in neurobiology to study vesicle fusion, neurotransmission, and synaptic plasticity.SNAP25 is encoded by the SNAP25 gene located on human chromosome 20p12.2. The protein is approximately 25 kilodaltons and anchored to the plasma membrane via palmitoylated cysteine residues in its central linker region. SNAP25 interacts with syntaxin and synaptobrevin (VAMP) to form the ternary SNARE complex, which brings vesicle and plasma membranes into close proximity for fusion. This complex enables rapid and precise neurotransmitter release at presynaptic terminals.The SNAP25 antibody detects a 25 kilodalton band by western blot and shows punctate presynaptic staining in neurons and neuroendocrine cells. SNAP25 participates in multiple exocytic events, including insulin secretion, catecholamine release, and synaptic vesicle recycling. It also regulates short-term synaptic plasticity by modulating vesicle pool dynamics.Phosphorylation of SNAP25 by protein kinases such as PKC and CDK5 fine-tunes its role in vesicle priming and fusion probability. Mutations or altered expression of SNAP25 are linked to neurological and psychiatric disorders, including attention deficit hyperactivity disorder, epilepsy, and schizophrenia. Reduced SNAP25 function leads to synaptic transmission defects and cognitive impairment.In addition to neurons, SNAP25 expression occurs in pancreatic beta-cells and endocrine tissues, reflecting its conserved role in vesicle exocytosis. NSJ Bioreagents provides a validated SNAP25 antibody optimized for western blot, flow cytometry, and neuronal culture staining, supporting detailed analysis of neurotransmitter release, synaptic machinery, and neurosecretory regulation.