Anti-SMTN / Smoothelin

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SMTN antibody recognizes Smoothelin, a cytoskeletal protein encoded by the SMTN gene and specifically expressed in contractile smooth muscle cells. Smoothelin is a key component of the actin cytoskeleton that contributes to the mechanical stability and contractile function of differentiated smooth muscle. This protein localizes to stress fibers and dense bodies, where it interacts with actin filaments to support force generation and cell shape maintenance. SMTN exists in multiple isoforms, including smoothelin-A, primarily found in visceral smooth muscle, and smoothelin-B, which is more abundant in vascular smooth muscle. The expression of these isoforms reflects the functional specialization of different smooth muscle types in the gastrointestinal tract, bladder, and blood vessels.Smoothelin is tightly regulated during the phenotypic switch between contractile and synthetic smooth muscle states. During vascular injury or atherosclerotic changes, smooth muscle cells often downregulate SMTN expression as they transition to a proliferative phenotype, highlighting its role as a marker of mature, differentiated smooth muscle. Experimental models have shown that SMTN interacts with alpha-actinin, calponin, and other structural proteins to form an integrated cytoskeletal network essential for contractility. The gene is located on chromosome 22q12.3 and is transcriptionally regulated by serum response factor (SRF) and myocardin-related transcription factors, both central regulators of smooth muscle differentiation.Immunohistochemical analysis reveals strong SMTN staining in vascular media, intestinal muscularis, and detrusor muscle, whereas non-muscle tissues lack expression. Because of its restricted distribution, SMTN antibody is widely used in smooth muscle cell research, vascular biology, and tumor pathology to distinguish contractile smooth muscle cells from fibroblasts or myofibroblasts. Loss or reduction of SMTN has been associated with vascular remodeling, hypertension, and certain myopathies. The smoothelin protein is also of interest as a potential biomarker for monitoring smooth muscle differentiation in stem cell-derived tissue engineering systems.SMTN antibody from NSJ Bioreagents can be applied in immunocytochemistry, immunohistochemistry, and other detection formats to study cytoskeletal organization and smooth muscle differentiation pathways in diverse model systems.