Anti-SMG8 / ABC2 / Nonsense-mediated mRNA decay factor SMG8

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SMG8 antibody detects Nonsense-mediated mRNA decay factor SMG8, encoded by the SMG8 gene on chromosome 17p13.3. SMG8 antibody is widely used in research on mRNA surveillance, nonsense-mediated mRNA decay (NMD), and translation regulation. SMG8 is part of the NMD pathway, which eliminates mRNAs containing premature stop codons to prevent production of truncated or potentially harmful proteins. SMG8, together with SMG9, regulates the kinase SMG1, a central factor in NMD signaling.Structurally, SMG8 is a ~113 kDa protein with HEAT repeat domains that mediate protein-protein interactions. It forms a stable heterodimer with SMG9, and together they act as regulatory subunits of the SMG1 kinase complex. This interaction modulates SMG1's ability to phosphorylate UPF1, the key effector protein in NMD. SMG8 is localized primarily in the cytoplasm, consistent with its role in mRNA surveillance during translation.Functionally, SMG8 inhibits SMG1 kinase activity until appropriate NMD activation signals occur. This regulation ensures phosphorylation of UPF1 only when premature termination codons are recognized. By controlling SMG1 activity, SMG8 fine-tunes the NMD pathway and prevents unnecessary degradation of functional mRNAs. Researchers use SMG8 antibody to study mRNA decay, translation fidelity, and quality control in gene expression.Clinically, mutations and dysregulation of SMG8 are associated with neurodevelopmental disorders and intellectual disability. Because NMD contributes to transcriptome regulation beyond error correction, alterations in SMG8 may influence disease-related gene expression. Abnormal NMD has been implicated in cancer, neurodegeneration, and genetic diseases, positioning SMG8 as a candidate biomarker. NSJ Bioreagents provides SMG8 antibody for studies of RNA surveillance, translation control, and disease mechanisms.Experimentally, SMG8 antibody is applied in western blotting to detect the ~113 kDa protein, in immunoprecipitation to isolate SMG1-SMG8-SMG9 complexes, and in immunofluorescence microscopy to study cytoplasmic distribution. Co-immunoprecipitation with SMG8 antibody identifies partners including SMG1, SMG9, and UPF1.