Anti-SMAD4, CT (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, De

SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Flow Cytometry and Immunofluorescence . Other applications not tested. Recommended Dilution: ELISA: 1:1,000 , Western Blot: 1:50-1:100, Immunohistochemistry: 1:50-1:100, Flow cytometry: 1:10~1:50, Immunofluorescence: 1:10~1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.