Anti-Smac/Diablo (human), Rabbit Monoclonal (RM271)

Recombinant Antibody. This antibody reacts to human Smac (Diablo homolog, mitochondrial). Applications: WB, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Smac/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis. Upon mitochondrial stress, Smac/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins). The interaction of Smac/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases. This interaction involves mainly the amino-terminal residues of Smac/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac/Diablo and other areas of BIR3. Furthermore, Smac/DIABLO plays an important regulatory role in the sensitization of cancer cells to both immune-and drug-induced apoptosis. It promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway and acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).