Anti-SK4 (Intermediate conductance Ca 2 -activated Potassium Channel 4, IK Ca 1, SK4, K Ca 3.1, KCNN

KCa 3.1 is part of the Ca 2+ activated K +channels family that share the characteristic of being activated by intracellular Ca 2+ . The channel has an intermediate conductance, is voltage insensitive and is activated by Ca 2+ in the submicromolar range. The channel has a similar topology to that of K Vchannels, that is six transmembrane domains and intracellular N- and C-termini. KCa 3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel. The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl -and Ca 2+. Indeed, K Ca 3.1 has a key role in sustaining the Ca 2+ influx in activated T lymphocytes and in regulating Cl -secretion from colon epithelium. Therefore, specific blockers of the K Ca 3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:300 using ECL on KCa 3.1 transfected HEK-293 and K562 cells (Human chronic myelogenous leukemia). Dilutions should be made using a carrier protein such as BSA (1-3%), Optimal dilutions to be determined by the researcher. Control Antigen (Supplied): S1014-09W1: SK4 Control Antigen (Intermediate conductance Ca 2+ -activated Potassium Channel 4, IK Ca 1, SK4, K Ca 3.1, KCNN4) , The stock solution of the antigen can be made up using 100ul of sterile ddH2O. For negative control, preincubate 1ug of peptide with 1ug of antibody for one hour at RT.. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.