Anti-Six-transmembrane epithelial antigen of the prostate-1 (STEAP1, six transmembrane epithelial an

STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40kD protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell-cell junctions between prostate secretory epithelium, and upregulated in prostate and some bladder, colon and ovarian cancers and Ewing's sarcomas. Human STEAP1 is 339aa in length. It contains a ferric oxidoreductase domain (aa119-265) that includes transmembrane sequences (# 2, 3, 4 and part of 5, out of 6 sequences). Two splice forms diverge at aa255, terminating after aa58 or 259. Over aa1- 71, human and mouse STEAP1 share 68% aa identity. , , Applications: , Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml, Western Blot: 1.0ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.