Anti-SIK1 / Salt-inducible kinase 1

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SIK1 antibody detects Salt-inducible kinase 1, a serine/threonine kinase that regulates energy metabolism, stress signaling, and transcriptional responses to hormonal cues. SIK1 belongs to the AMP-activated protein kinase (AMPK) family and is activated by LKB1-mediated phosphorylation. The SIK1 antibody is commonly used to investigate glucose metabolism, circadian rhythm, and stress adaptation in mammalian cells.SIK1 is encoded by the SIK1 gene located on human chromosome 21q22.3. The protein is approximately 78 kilodaltons and consists of an N-terminal kinase domain, an autoinhibitory sequence, and a C-terminal region rich in phosphorylation sites. SIK1 modulates transcription by phosphorylating CREB-regulated transcription coactivators (CRTCs), leading to their cytoplasmic retention and suppression of CREB-dependent gene expression.The SIK1 antibody detects a strong 85 kilodalton band by western blot. Under stress or hormonal stimulation, SIK1 undergoes rapid induction and nuclear translocation. It acts as a feedback regulator controlling CREB activity, gluconeogenic gene expression, and circadian rhythm stability.Functionally, SIK1 plays an important role in metabolic homeostasis. It regulates hepatic glucose output, lipid metabolism, and skeletal muscle adaptation to exercise. In the brain, SIK1 contributes to neuronal plasticity and sleep-wake regulation. Dysregulation of SIK1 expression or phosphorylation has been implicated in hypertension, metabolic syndrome, and mood disorders.SIK1 is a key mediator of hormonal signaling via cAMP and glucocorticoids. It interacts with 14-3-3 proteins and phosphatases, allowing dynamic control of kinase activity in response to changing energy states. NSJ Bioreagents provides a validated SIK1 antibody optimized for its applications, supporting exploration of metabolic regulation, transcriptional control, and stress physiology.