Anti-SHANK2 / SH3 and multiple ankyrin repeat domains protein 2

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SHANK2 antibody detects SH3 and multiple ankyrin repeat domains protein 2, a scaffolding protein of the postsynaptic density (PSD) that organizes synaptic signaling complexes. The UniProt recommended name is SH3 and multiple ankyrin repeat domains protein 2 (SHANK2). This large multidomain protein serves as a structural hub connecting neurotransmitter receptors, ion channels, and cytoskeletal elements at excitatory synapses.Functionally, SHANK2 antibody recognizes a synaptic scaffolding protein of approximately 180-200 kDa that coordinates synaptic architecture and signaling. SHANK2 interacts with PSD-95, Homer, and cortactin, linking surface glutamate receptors to the actin cytoskeleton. These interactions stabilize dendritic spine morphology and facilitate efficient synaptic transmission. SHANK2's PDZ, SAM, and ankyrin repeat domains enable it to integrate diverse protein-protein interactions, forming large macromolecular complexes essential for signal transduction.The SHANK2 gene is located on chromosome 11q13.3 and encodes multiple isoforms through alternative splicing. SHANK2 is particularly abundant in the cerebral cortex, hippocampus, and cerebellum, where it regulates synaptic plasticity and neuronal connectivity. Genetic studies associate SHANK2 mutations and deletions with autism spectrum disorder (ASD), intellectual disability, and schizophrenia, linking its dysfunction to altered excitatory signaling and neural circuit development.SHANK2 antibody is a powerful tool for neuroscience research, enabling investigation of postsynaptic organization, receptor clustering, and synaptic remodeling. It is widely applied in western blotting, immunofluorescence, and co-immunoprecipitation to study excitatory synapses and protein interactions within the PSD. In neuronal cultures, SHANK2 colocalizes with synaptic markers such as PSD-95 and Synapsin, marking mature excitatory synapses. Loss of SHANK2 expression disrupts dendritic spine density and reduces AMPA and NMDA receptor-mediated currents, impairing synaptic strength.Structurally, SHANK2's N-terminal ankyrin repeats mediate cytoskeletal binding, while its C-terminal SAM domain enables oligomerization, forming mesh-like scaffolds beneath the postsynaptic membrane. Post-translational modifications, including phosphorylation and palmitoylation, regulate its localization and synaptic activity. SHANK2 also interacts with neuroligins and mGluR5, connecting receptor signaling to structural reorganization. Dysregulation of SHANK2 leads to impaired synaptic signaling and behavioral abnormalities in animal models.NSJ Bioreagents provides SHANK2 antibody reagents validated for research use in neurobiology, synaptic physiology, and disease modeling. These antibodies are ideal for identifying postsynaptic protein networks and assessing synaptic architecture in normal and pathological conditions.