Anti-SERPINB12 / Serpin family B member 12

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SERPINB12 antibody targets Serpin family B member 12, a cytoplasmic serine protease inhibitor belonging to the clade B (ovalbumin-like) subfamily of serpins. These intracellular serpins modulate proteolytic activity to maintain cellular homeostasis, control inflammation, and prevent premature cell death caused by uncontrolled protease action. SERPINB12, encoded by the SERPINB12 gene on chromosome 18q21.33, is expressed in epithelial tissues such as the liver, pancreas, kidney, and gastrointestinal tract. The protein functions primarily as an inhibitor of trypsin-like serine proteases, including granzyme B and trypsin, protecting cells from protease-mediated damage during stress or immune response.Structurally, SERPINB12 consists of a conserved serpin fold comprising three beta-sheets and nine alpha-helices surrounding a reactive center loop (RCL). Upon cleavage by a target protease, SERPINB12 undergoes an irreversible conformational change that traps the enzyme in a covalent complex, neutralizing its activity. This suicide-inhibition mechanism is characteristic of the serpin family and critical for regulating proteolytic cascades within cells. In the pancreas and gastrointestinal tract, SERPINB12 prevents inappropriate activation of digestive enzymes that could cause tissue injury. In immune contexts, it protects epithelial cells against cytotoxic proteases released by immune effectors.Beyond its role in protease inhibition, SERPINB12 has emerging significance in cancer biology and tissue homeostasis. Overexpression of SERPINB12 has been reported in hepatocellular carcinoma, pancreatic ductal adenocarcinoma, and colorectal cancer, where it may contribute to cell survival under inflammatory or proteolytic stress. Conversely, loss of SERPINB12 function has been associated with enhanced susceptibility to apoptosis and tissue injury. The SERPINB12 antibody is widely used in studies examining protease regulation, epithelial defense, and tumor biology. Immunohistochemistry with this antibody reveals cytoplasmic localization consistent with its role as an intracellular serpin. In biochemical assays, western blotting identifies a 42 kilodalton band corresponding to SERPINB12, confirming its specificity.Functional studies have shown that SERPINB12 may cooperate with other serpins, such as SERPINB9 and SERPINB13, to establish a network of intracellular protease control in secretory epithelia. This redundancy ensures protection against multiple classes of serine proteases during inflammation or infection. In model systems, SERPINB12 expression is upregulated by cytokines and growth factors, suggesting regulation through stress-responsive pathways. The SERPINB12 antibody thus provides a valuable tool for mapping these protective responses and for characterizing epithelial resilience to proteolytic challenge. Its expression profile also makes it a potential biomarker for epithelial tumor progression and inflammation-associated pathology. NSJ Bioreagents supplies this antibody validated for its applications, supporting diverse studies of serpin-mediated protease regulation.