Anti-Serotonin Transporter (SERT)

Serotonin (5-hydroxytryptamine), MW 176, is a catecholamine , a monoamine neurotransmitter, found in abundance in the central nervous system, platelets, brain and spinal cord as well as secreted by enterochromaffin cells (EC or Kulchitsky cells, a type of enteroendocrine cell occurring in the epithelia lining the lumen of the gastrointestinal tract) in the stomach, intestines, appendix and in endocrine cells along the gut and in carcinoid tumors. Platelets and mast cells are rich in serotonin. Serotonin plays important physiological functions at the intestinal level. Human intestinal epithelial Caco-2 cellls functionally express SERT both at their apical and basolateral cell membranes. It is a 630aa residue in human (chromosome 17q11), rat and mouse. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested., , Recommended Dilution: , Western Blot: 1:1000, Immunohistochemistry (Cy3 technique): 1:800-1:1000 in PBS, 0.3% Triton X-100., Immunohistochemistry (Biotin/avidin-HRP): 1:10,000-1:15,000 in PBS, 0.3% Triton X-100 ., Optimal dilutions to be determined by the researcher. Positive Control Tissue: Rat raphe nuclei, hypothalamus, cortex and spinal cord, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.