Anti-Semaphorin 4G (SEMA4G, KIAA1619)

Semaphorin 4G is a 92kD (predicted), class IV member of the semaphorin family of proteins. It is expressed by select cell types, including oligodendroglia and Purkinje neurons, and likely acts as a chemorepellent by binding to B-type plexins. Mature mouse Sema4G is a type I transmembrane protein that is 820aa in length. It contains a 656aa extracellular region aa18-673 that is characterized by one Sema domain aa35-503, a PSI region aa505-556 and an Ig-like C2-type domain aa565-648. The 143aa cytoplasmic region contains a poly-proline segment and a Ser phosphorylation site. Over aa 18-673, mouse Sema4G shares 91-97aa identity with human and rat Sema4G, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.