Anti-SEC63 / DNAJC23

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SEC63 antibody detects Sec63 homolog, a component of the endoplasmic reticulum (ER) translocon complex encoded by the SEC63 gene on chromosome 6q21. SEC63 is an integral membrane protein essential for protein translocation into the ER and is conserved from yeast to humans. It functions as part of the SEC61/SEC62/SEC63 complex, which mediates post-translational import of secretory and membrane proteins into the ER lumen. SEC63 interacts directly with BiP (HSPA5), the ER-resident chaperone, to facilitate ATP-driven polypeptide movement across the translocon pore. The presence of SEC63 ensures proper folding and maturation of newly synthesized proteins, thereby maintaining proteostasis and ER homeostasis.Structurally, SEC63 is a multi-pass membrane protein with a luminal J-domain that binds BiP. This J-domain stimulates BiP's ATPase activity, coordinating polypeptide translocation and preventing premature folding. SEC63 also contains hydrophobic transmembrane segments that anchor it within the ER and permit association with SEC61 alpha, beta, and gamma subunits. Loss-of-function mutations in SEC63 disrupt ER import, leading to protein misfolding and accumulation, hallmarks of ER stress and unfolded protein response activation.Clinically, SEC63 is associated with polycystic liver disease (PCLD). Mutations in SEC63 impair secretory protein maturation, particularly affecting hepatocyte glycoproteins, leading to cyst formation and progressive liver dysfunction. Animal models with SEC63 deficiency replicate these phenotypes, confirming its pathogenic role. Moreover, SEC63 has been implicated in cancer biology: dysregulated expression alters secretory pathways and contributes to tumor growth, metastasis, and altered stress signaling. SEC63 antibody is therefore a valuable research tool in hepatology, ER biology, and cancer studies.Experimentally, SEC63 antibody is used for western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. In cultured cells, immunostaining shows SEC63 localization in the perinuclear ER network, while biochemical assays reveal its incorporation into SEC61 complexes. Researchers employ SEC63 antibody to investigate ER import fidelity, stress responses, and disease-related mutations. In clinical research, SEC63 detection provides insights into genetic causes of liver cysts and potential therapeutic approaches targeting protein folding pathways. NSJ Bioreagents provides SEC63 antibody to ensure consistent, validated detection for these applications.