Anti-SCAMP3 (secretory carrier membrane protein 3, Propin 1, C1orf3, PROPIN1, Secretory carrier)

SCAMP3 (Secretory carrier membrane protein 3) is a 38-40kD member of the SCAMP , family of proteins. It is expressed in heart and skeletal muscle, plus fat, and functions in , post-Golgi recycling and EGFR internalization pathways. Human SCAMP3 is 347aa in length. It contains a cytoplasmic N-terminus (aa1-170) that shows three NPF repeats (binding sites for EH domain proteins), a heptad repeat of aliphatic aa's, and a potential SH3-binding motif. This is followed by four consecutive transmembrane domains (aa171-297) plus a C-terminal cytoplasmic region (aa298-347). It appears that phosphorylation of SCAMP3 on Tyr41 is a requisite for its interaction with EGFR. There are three potential splice forms. One shows a deletion of aa 23-48, while two others show a one and 23 aa substitution for aa131-144 and 131-260, respectively. Over aa1-170, human and mouse SCAMP3 are 91% aa identical. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1.0ug/ml , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.