Anti-SASS6 / Spindle assembly abnormal protein 6

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. SASS6 antibody recognizes Spindle assembly abnormal protein 6 homolog, a centriolar protein required for centriole duplication and proper cell division. Encoded by the SASS6 gene on chromosome 1p21.2, this protein is a structural component of the cartwheel, a ninefold symmetric scaffold that initiates centriole assembly. SASS6 self-oligomerizes through its coiled-coil domain to establish the radial symmetry of nascent centrioles, ensuring accurate duplication and inheritance of centrosomes during each cell cycle. The protein localizes to the proximal ends of centrioles and is essential for maintaining centrosome integrity and mitotic spindle organization.Loss of SASS6 disrupts centriole formation, resulting in monopolar spindles and abnormal cell division. Mutations in SASS6 cause primary microcephaly, characterized by reduced brain size and defective neural progenitor proliferation. The SASS6 antibody allows detection of centriolar structures in cultured cells and tissue samples. Immunofluorescence using this antibody reveals punctate staining at centrosomes during interphase and mitosis, while western blotting identifies a 65 kilodalton band. Because of its critical role in centrosome biogenesis, SASS6 serves as a core marker for centriole assembly studies and cell cycle regulation research.Beyond its role in structural assembly, SASS6 interacts with STIL and CEP135 to coordinate recruitment of microtubule-nucleating proteins. Its regulated degradation through the APC/C complex ensures centriole duplication occurs only once per cell cycle. Overexpression of SASS6 leads to centrosome amplification, a phenotype frequently observed in cancer cells. The SASS6 antibody is thus valuable for examining centrosome abnormalities and chromosomal instability associated with tumorigenesis. Researchers also employ this antibody in developmental studies to trace centriole formation during early embryogenesis. NSJ Bioreagents provides a validated SASS6 antibody for use in immunofluorescence, western blot, and cell imaging assays focused on centrosome dynamics and mitotic control.